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. 2021 Nov 23;13(23):5881. doi: 10.3390/cancers13235881

Figure 4.

Figure 4

Detection of rVAR2-captured cancer cells by RT-qPCR. (a) cDNA strand count for USH1C (black circles) and CKMT1A (grey squares) detected after capture of 100 COLO205 cells from 1 mL blood using the rVAR2-approach. Beads were either removed prior to cell lysis by trypsinization, removed directly from the lysed product or left in the lysed product (no removal). Lines represent the mean. (b) cDNA strand count for USH1C detected after spike-in of 50, 25, 10 or 0 COLO205 cells (closed circles) or SW480 cells (open circles) to 3 mL blood (each test was performed in duplicate). Cells were captured with rVAR2 via magnetic beads, and RNA transcripts were quantified by RT-qPCR. Lines represent the mean. (c) As in (b) but for CKMT1A. (d) Number of detected cDNA strands of USH1C after rVAR2 capture of 10 or 0 cells from 3 mL blood. Each type of symbol represents an individual experiment (n = 4) always carried out in duplicates. Data from the COLO205 experiment shown in (b,c) is included as a 4th replicate. Lines represent median. 0 cells vs 10 cells were compared statistically using a Mann-Whitney test. (e) As in (e) but for CKMT1A.