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. 2021 Nov 23;13(23):5873. doi: 10.3390/cancers13235873

Figure 5.

Figure 5

Three-dimensional culture of 786-O utilizing tumor-specific ECM. (A–C) Modification of fibrinogen concentrations, 2 µg/mL, 4 µg/mL, and 10 µg/mL changes morphology of cell structures formed by 786-O cells. Yellow dotted line outlines region containing 3D dome. Red dotted line indicates region of magnified inset. (D–F) Live–dead analysis of cell structures found in fibrin domes with 4 mg/mL fibrinogen shows viable elongated structures (E) as well as ones with necrotic cores (F). Arrowhead indicates viable cells. Arrow indicates dead cells. Blue = Hoechst33342, green = calcein-AM, red = ethidium homodimer 2. (G) Analysis of spheroids in Matrigel or fibrin with or without added ccRCC ECM proteins showing changes in number of spheroids and size. (H,I) Example of 786-O growth in Matrigel versus fibrin mixed with collagen VI (additional single ECM component blends with fibrin are shown in Figure S6). Yellow dotted line outlines region containing 3D dome.