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. 2021 Nov 26;13(23):5946. doi: 10.3390/cancers13235946

Figure 2.

Figure 2

CARMA3 expression is critical for CRC’s aggressive abilities. (A) The basal expression level of CARMA3 in SW480, SW620, HT-29, and HCT116 cells. α-Tubulin was used as the internal protein loading control. (B) Overexpression efficiencies of CARMA3 were examined by Western blotting in both SW480 and HT-29 cells. Ctrl represented as control cells. (C) Proliferation curves were analyzed in both SW480 and HT-29 cells with CARMA3-overexpressed and control cells for four days by MTT assay. Proliferation rates were significantly higher in CARMA3-overexpressed cells compared to control cells. *, p < 0.05 (Student’s t-tests). (D,E) Both transwell migration and invasion assays of CARMA3 overexpression in SW480 and HT-29 cells were shown. Results are shown as the mean ± S.D. of three independent experiments. *, p < 0.05 (Student’s t-tests). Scale bar, 100 μm. (F) Knockdown efficiencies of CARMA3 by shRNAs of two clones in both SW620 and HCT116 cells were analyzed by Western blotting. (G,H) Both transwell migration and invasion abilities were determined in CARMA3-knocked down cells compared to their shRNA control (shCtrl) cells. Results are shown as the mean ± S.D. of three independent experiments. *, p < 0.05. Magnification: 40×. Scale bar, 100 μm.