Figure 4.

Effects of the rs6086746 genotype on luciferase activity in cultured HEK293cells. HEK293 cells were transfected with 0.5 μg pGL3 basic-LUC luciferase reporter recombinant plasmids containing a PLCB4 promoter sequence with the wild-type G allele or A allele at the rs6086746 SNP in the presence of 0.5 μg pSG5.HA control vector or pSG5.HA.RUNX2 vector. Transfected cells were cultured for 24 h. Luciferase activity in cell extracts was expressed in relative light units (RLUs). Mean ± SEM is given for each construct from three experiments. *p < 0.05.