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. 2021 Nov 30;22(23):12961. doi: 10.3390/ijms222312961

Figure 2.

Figure 2

Primaquine has an impact on the endosome system and impairs the endocytosis-mediated degradation of EGFR. (A) MDA-MB-231 and MDA-MB-453 cells were exposed to DMSO or 50 μM primaquine for 1 h before processing for immunofluorescence microscopy and stained with anti-EEA1 and DAPI. (B) MDA-MB-231 and MDA-MB-453 cells were exposed to 50 µM primaquine or DMSO for 1 day and then treated with EGF from 0 to 60 min. Before processing for immunofluorescence microscopy, cancer cells were stained with anti-EEA1 and anti-EGFR. Insets represent high magnification images of the region indicated by the yellow rectangle.