Figure 6.

The effects of losartan on the expression of genes and proteins related to the cardiac renin-angiotensin-aldosterone system and canonical SMAD-dependent pathway at weeks 1, 3, and 15. Left ventricular expression of (a) angiotensinogen (Agt) normalized to ribosomal protein lateral stalk subunit P2 (RpIp2) gene expression, left ventricular protein expression and cropped representative images of (b) angiotensin II type 1 receptor (AT1R, 41 kDa), and (c) angiotensin II type 2 receptor (AT2R, 41 kDa), (d) left ventricular expression of transforming growth factor-beta (Tgfb) normalized to RpIp2 gene expression, left ventricular expression and cropped representative images of (e) TGF-β receptor II (TGF-βRII, 85 kDa) and (f) SMAD2/3 (52 and 60 kDa). Values are presented as mean ± S.E.M., * p < 0.05 vs. control group, # p < 0.05 vs. RT only group (n = 6–7, One-Way ANOVA, Holm-Sidak post hoc test). Ctrl: control group, RT: radiotherapy only group (50 Gy), RT Los: RT plus losartan group. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 37 kDa) was used as a loading control in protein expression changes assessed by Western blot. Images were captured with the Odyssey CLx machine and exported with Image Studio 5.2.5 software. Black lines next to the Western blot images represent the position of protein markers with corresponding molecular weights. The uncropped Ponceau-stained membranes and the full-length Western blot images with the protein ladders are presented in Figures S1–S9.