Figure 2.

PII foci formation in Arabidopsis thaliana under different temperature and light regimes. Expression of genomic AtPII-GFP under the control of the pUBQ10 promotor (pUBQ10::AtPII_cDNA-GFP) was analyzed in 6-day-old seedlings. Seedlings were incubated for 24 h for temperature treatment in the dark at (A) RT, (B) 8 °C, (C) 37 °C, and for light treatment in (D) blue light, (E) green light, (F) red light, and (G) far red light. Seedlings were fixed after incubation. In each row, the GFP fluorescence is shown first, chlorophyll autofluorescence is shown second, and the merge of both pictures is shown last. White arrows: PII foci. Scale bar: 10 µm.