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. 2021 Nov 30;22(23):12985. doi: 10.3390/ijms222312985

Figure 2.

Figure 2

RYR1 plays a key role during early human myoblast differentiation. (A) RYR1 mRNA expression levels were assessed in control (n = 5) and DMD (n = 6) myoblasts at confluence and in myotubes at day 3–4 of differentiation by quantitative RT-PCR. (B) Representative immunofluorescence images of human control myotubes in which RYR1 was silenced or not (scramble). Myoblasts at 80% of confluence (n = 5) were incubated with RYR1-specific or scramble siRNAs and then grown in differentiation medium for 3–4 days. Myotubes were stained for troponin T (green) and DAPI (blue) to determine myotube morphology using three parameters (C): total myotube area (cells with at least 3 merged nuclei), total number of nuclei and fusion index (nuclei per myotube/total nuclei). * p < 0.05.