Figure 5.

Endomysial fibrosis is correlated with elevated resting intracellular calcium concentration. (A,B) Representative images showing color segmentation of muscle sections stained with Masson trichrome (left) and with pseudo-color (yellow) to highlight endomysial fibrosis (right) in one healthy control muscle biopsy (A) with minimal (1.7%) endomysial fibrosis (biopsy of a 10-year-old boy) and one DMD muscle biopsy (B) with advanced (24%) endomysial fibrosis (biopsy of a 5-year-old boy). (C) Quantification of endomysial fibrosis in muscle biopsies of healthy controls (n = 5) and patients with DMD (n = 6). ** p < 0.01. (D) Correlation between the endomysial fibrosis rate and the Fura-2 ratio at rest in patients with DMD (n = 6, triangles) and healthy controls (n = 5, circles) (Spearman’s rank correlation coefficient r = 0.56, p < 0.01).