Figure 5.

The w¹¹¹⁸ mutants have increased freeze tolerance. Wildtype Canton-S (w⁺), w¹¹¹⁸, and w¹¹¹⁸; NP1131(mini-white) genotypes were subjected to 120 min at −5 °C, followed by a 24 h recovery at 25 °C. The median percentage of mortality after this recover period is shown as the center line, with the mean indicated by the x. There were significant differences in mortality between genotypes (Kruskal–Wallis, k = 73.404, p < 0.0001, n = 64–68). The w¹¹¹⁸ flies survived the subfreezing temperature significantly better than w⁺ and w¹¹¹⁸; NP1131 (Dunn’s procedure, p < 0.0001; n = 64–68). Groups that do not share letters above the box plots are significantly different from each other according to the Dunn’s post hoc test (p < 0.05).