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. 2021 Nov 26;22(23):12827. doi: 10.3390/ijms222312827

Figure 3.

Figure 3

OD depends on cell seeding number/density, MTT concentration, and incubation time: OD changes with increasing MTT incubation time. (a) OD of cell-free culture media (Phenol red-containing RPMI + 10% FCS) at different time points after incubation with different concentrations of MTT. (b–d) OD of PC-3 cells with different seeding numbers of 5000 (b), 10,000 (c), and 20,000 (d) per well of 96-well plates. Cells were allowed to grow for 26 h before the addition of different concentrations of MTT and the OD was measured at different time points following MTT addition. Data shown as mean OD of triplicate wells and error bars represent SD (standard deviation).