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. 2021 Nov 26;22(23):12827. doi: 10.3390/ijms222312827

Figure 4.

Figure 4

OD depends on cell seeding number/density, MTT concentration, and incubation time: OD changes with increasing MTT incubation time. PC-3 cells were allowed to grow in 96-well plates for 26 h before incubation with MTT. OD was measured at different time points after addition of 10 µL of (a) PBS, or (b) 0.1 mg/mL, (c) 0.2 mg/mL, (d) 0.3 mg/mL, (e) 0.4 mg/mL, and (f) 0.5 mg/mL of MTT–PBS solutions to 100 µL of each well content. Data shown as mean OD of triplicate wells and error bars represent standard deviation (SD).