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. 2021 Nov 26;22(23):12827. doi: 10.3390/ijms222312827

Figure 6.

Figure 6

OD depends on cell seeding number/density, MTT concentration, and incubation time: OD changes with increasing MTT concentration. PC-3 cells were seeded at different numbers in 96-well plates and were allowed to grow for 26 h before adding 10 µL MTT–PBS solution of different concentrations (0–0.5 mg/mL) to 100 µL of each well content. OD was measured following 2 h (a), 3 h (b), and 4 h (c) of incubating cells with MTT. Data shown as mean OD of triplicate wells and error bars represent SD (standard deviation).