Figure 4.

Knockdown of Zeb1 in PC-3 prostate cancer cells leads to a partial-EMT phenotype at the cellular and molecular level. (A,B) Cultured PC-3 Zeb1^KD (Zeb1 knockdown) and control (ctrl) cells were assessed for cell morphology characteristics as described in the Materials & Methods and in Supplementary Figure S3 (N = 3; n = 250/cells per group). Representative images of each cell group and epithelial (MDA-MB-468) and mesenchymal (primary lung fibroblasts) controls are shown. (C) Immunoblot analysis of P-Cadherin and ITGβ4 in Zeb1^KD or ctrl cells. Actin was used as a loading control and representative immunoblots are shown. (D) qRT-PCR analysis of p-EMT marker expression in the presence of absence of Dox in Zeb1^KD or ctrl cells. Data is presented as the mean ± standard error of the mean (SEM) (n = 3) relative to ctrl no Dox. Scale bars = 50 µm. α = significantly different than PC-3 ctrl no Dox. β = significantly different than ctrl with Dox. δ = significantly different than Zeb1^KD no Dox (p ≤ 0.05).