Figure 3.

CXCL9 drives dose-dependent increased chemotaxis of T cell subsets in Gba1^9V/− mice. Spleen-derived CD4⁺ T cells from WT and Gba1^9V/− mice (n = 5/group) were allowed to migrate towards different concentrations of CXCL9 (0, 2, 4, 8, and 16 nM) at 37 °C and 5% CO₂ for 45 min. The migration membrane was removed and stained with Diff-Quick and cells were counted under the light microscope. Diff quick- images and the corresponding bar diagrams represent the CXCL9 induced T cells migration in WT (a–c) and Gba1^9V/− mice (b,c). WT (black curve), Gba1^9V/− (maroon curve) and values shown are the mean ± SD. and group comparison were performed with ANOVA (ns, not significant; **, p < 0.01; ****, p < 0.0001).