Figure 4.

CXCR3 targeting alters CXCL9-mediated CD4⁺ T cells chemotaxis in Gba1^9V/− mice. CD4⁺ T cells purified from spleen of WT and Gba1^9V/− mice (n = 5/group) were allowed to migrate towards CXCL9 (16 nM) in the presence and absence of mouse anti-CXCR3 antibodies (10 μg/mL) at 37 °C and 5% CO₂ for 45 min. Cells that had migrated through the filter and had attached to the lower side of the filter were collected and analyzed by FACS. Percentage of CD4⁺CD11b⁻ T cells are shown from the (a) vehicle (PBS) treated WT cells and their migration to CXCL9, (b) mouse anti-CXCR3 antibodies treated WT cells and their migration to CXCL9, (c) PBS treated Gba1^9V/− cells and their migration to CXCL9, and (d) mouse anti-CXCR3 antibodies treated Gba1^9V/− cells and their migration to CXCL9. (e) WT (black columns), Gba1^9V/− (maroon columns) and the values shown in the bar diagram are the mean ± SD. and group comparison were performed with ANOVA. Three independent experiments were conducted (**** p < 0.0001).