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. 2021 Nov 24;22(23):12707. doi: 10.3390/ijms222312707

Figure 10.

Figure 10

HbSnRK2.6A and HbSnRK2.6B are direct transcriptional activators of HbICE2: (A) Schematic diagrams of effector and reporter constructs for dual-LUC transient expression assay; (B,C) relative LUC activity of HbCBF1 promoter in tobacco leaves with transient expression of 35S::HbICE2, 35S::HbICE2 + 35S::HbSnRK2.6s. The Agrobacterium tumefaciens GV3101 harboring HbCBF1::LUC and other effector plasmids were transferred into tobacco leaves, leaves transfected with HbCBF1::LUC only were used as a control. After infiltration for 48 h, the infected tobacco plants were exposed to 4 °C for 4 h, and then relative LUC activity of HbCBF1 promoter was quantified by measuring relative LUC and REN activities. Data shown are mean ± SD of three independent experiments, and asterisks indicate significant differences at ** p < 0.01 and *** p < 0.001 (Student’s t-test).