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. 2021 Nov 25;22(23):12737. doi: 10.3390/ijms222312737

Figure 7.

Figure 7

The FAK/AKT/HIF-1α signaling pathway is involved in CCL4-downregulated miR-3927-3p expression. (A) 143B cells were treated for 30 min with a FAK i (10 μM), AKT i (10 μM), or HIF i (10 μM) then incubated with CCL4 for 24 h. miR-3927-3p expression was examined by qPCR. (B) The wild-type (WT) or mutant binding sites (MUT) of ITGAV and ITGB3 3′UTRs containing the miR-3927-3p binding site were inserted into the pmirGLO vector. (C,D) The WT- or MUT-3′UTR luciferase activities of integrin αv and β3 were measured after MVC treatment or transfection with miR-3927-3p mimic. (E) miR-3927-3p expression in normal bone and osteosarcoma tissues were detected by qPCR. (F) Analysis of the correlation between CCL4 and miR-3927-3p expression in osteosarcoma tissue. The results were obtained from three independent experiments and are expressed as the means ± SD. * p < 0.05 compared with controls; # p < 0.05 compared with CCL4-treated controls.