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Validation of the hub genes in clinical tissues (A–C) Kaplan–Meier survival curves for patients of GBM with high and low gene expression in the TCGA dataset. (D–F) qRT-PCR of KRT8, NGFR, and TCEA3 in clinical human GBM samples and normal brain tissues. The expression levels were normalized to β-actin. ***P < 0.001. (G–I) The protein expression of KRT8, NGFR, and TCEA3 in clinical human GBM tissues and normal tissues was detected by WB.
