Table 1.
Association study with newborn IFNAR1 and IFNL SNPs and CZS abnormalities.
| No CZS findings | CZS | Adjusted by trimester of ZIKV exposure and ancestry | |||||||
|---|---|---|---|---|---|---|---|---|---|
| N | % | N | % | OR | lower | upper | p-value | FDR p-value | |
| rs2257167 | |||||||||
| G/G | 56 | 73.7 | 38 | 58.5 | 1 | 0.023 | 0.068 | ||
| C/G | 18 | 23.7 | 24 | 36.9 | 3.13 | 1.19 | 8.21 | ||
| C/C | 2 | 2.6 | 3 | 4.6 | 5.72 | 0.77 | 42.47 | ||
| G/G | 56 | 73.7 | 38 | 58.5 | 1 | 0.007 | 0.032 | ||
| C/G-C/C | 20 | 26.3 | 27 | 41.5 | 3.41 | 1.35 | 8.6 | ||
| G/G-C/G | 74 | 97.4 | 62 | 95.4 | 1 | 0.171 | 0.308 | ||
| C/C | 2 | 2.6 | 3 | 4.6 | 3.83 | 0.55 | 26.97 | ||
| G/G-C/C | 58 | 76.3 | 41 | 63.1 | 1 | 0.032 | 0.072 | ||
| C/G | 18 | 23.7 | 24 | 36.9 | 2.71 | 1.07 | 6.92 | ||
| Additive | 76 | 53.9 | 65 | 46.1 | 2.76 | 1.29 | 5.91 | 0.006 | 0.032 |
| rs2843710 | |||||||||
| G/G | 30 | 40 | 24 | 36.4 | 1 | 0.210 | 0.629 | ||
| C/G | 36 | 48 | 27 | 40.9 | 1.15 | 0.47 | 2.82 | ||
| C/C | 9 | 12 | 15 | 22.7 | 2.65 | 0.85 | 8.31 | ||
| C/C | 30 | 40 | 24 | 36.4 | 1 | 0.340 | 0.765 | ||
| C/G-G/G | 45 | 60 | 42 | 63.6 | 1.49 | 0.65 | 3.41 | ||
| C/C-C/G | 66 | 88 | 51 | 77.3 | 1 | 0.082 | 0.525 | ||
| G/G | 9 | 12 | 15 | 22.7 | 2.46 | 0.87 | 6.92 | ||
| C/C-G/G | 39 | 52 | 39 | 59.1 | 1 | 0.641 | 1.000 | ||
| C/G | 36 | 48 | 27 | 40.9 | 0.83 | 0.37 | 1.85 | ||
| Additive | 75 | 53.2 | 66 | 46.8 | 1.55 | 0.89 | 2.69 | 0.117 | 0.525 |
| rs2834202 | |||||||||
| A/A | 44 | 58.7 | 40 | 62.5 | 1 | 0.398 | 1 | ||
| A/G | 29 | 38.7 | 20 | 31.2 | 0.87 | 0.37 | 2.04 | ||
| G/G | 2 | 2.7 | 4 | 6.2 | 3.26 | 0.48 | 21.89 | ||
| A/A | 44 | 58.7 | 40 | 62.5 | 1 | 0.923 | 1 | ||
| A/G-G/G | 31 | 41.3 | 24 | 37.5 | 1.04 | 0.46 | 2.33 | ||
| A/A-A/G | 73 | 97.3 | 60 | 93.8 | 1 | 0.186 | 1 | ||
| G/G | 2 | 2.7 | 4 | 6.2 | 3.41 | 0.52 | 22.42 | ||
| A/A-G/G | 46 | 61.3 | 44 | 68.8 | 1 | 0.605 | 1 | ||
| A/G | 29 | 38.7 | 20 | 31.2 | 0.8 | 0.35 | 1.85 | ||
| Additive | 75 | 54 | 64 | 46 | 1.21 | 0.62 | 2.36 | 0.567 | 1 |
| rs17875834 | |||||||||
| C/C | 72 | 93.5 | 56 | 88.9 | 1 | 0.162 | 0.729 | ||
| C/T | 5 | 6.5 | 7 | 11.1 | 2.76 | 0.63 | 12.06 | ||
| Additive | 77 | 55 | 63 | 45 | 2.76 | 0.63 | 12.06 | 0.162 | 0.729 |
| rs12979860 | |||||||||
| C/C | 31 | 40.3 | 22 | 33.3 | 1 | 0.608 | 1 | ||
| C/T | 34 | 44.2 | 28 | 42.4 | 1.3 | 0.54 | 3.15 | ||
| T/T | 12 | 15.6 | 16 | 24.2 | 1.78 | 0.56 | 5.68 | ||
| C/C | 31 | 40.3 | 22 | 33.3 | 1 | 0.406 | 1 | ||
| C/T-T/T | 46 | 59.7 | 44 | 66.7 | 1.42 | 0.62 | 3.25 | ||
| C/C-C/T | 65 | 84.4 | 50 | 75.8 | 1 | 0.419 | 1 | ||
| T/T | 12 | 15.6 | 16 | 24.2 | 1.54 | 0.54 | 4.38 | ||
| C/C-T/T | 43 | 55.8 | 38 | 57.6 | 1 | 0.858 | 1 | ||
| C/T | 34 | 44.2 | 28 | 42.4 | 1.08 | 0.48 | 2.39 | ||
| Additive | 77 | 53.8 | 66 | 46.2 | 1.33 | 0.76 | 2.33 | 0.319 | 1 |
| rs8099917 | |||||||||
| T/T | 58 | 75.3 | 43 | 65.2 | 1 | 0.309 | 0.695 | ||
| G/T | 17 | 22.1 | 18 | 27.3 | 2.09 | 0.78 | 5.61 | ||
| G/G | 2 | 2.6 | 5 | 7.6 | 1.66 | 0.27 | 10.11 | ||
| T/T | 58 | 75.3 | 43 | 65.2 | 1 | 0.130 | 0.542 | ||
| G/T-G/G | 19 | 24.7 | 23 | 34.8 | 2 | 0.81 | 4.95 | ||
| T/T-G/T | 75 | 97.4 | 61 | 92.4 | 1 | 0.706 | 1 | ||
| G/G | 2 | 2.6 | 5 | 7.6 | 1.41 | 0.23 | 8.44 | ||
| T/T-G/G | 60 | 77.9 | 48 | 72.7 | 1 | 0.154 | 0.542 | ||
| G/T | 17 | 22.1 | 18 | 27.3 | 2.02 | 0.76 | 5.41 | ||
| Additive | 77 | 53.8 | 66 | 46.2 | 1.63 | 0.78 | 3.37 | 0.181 | 0.542 |
| rs8109886 | |||||||||
| A/A | 24 | 31.2 | 22 | 33.3 | 1 | 0.795 | 1 | ||
| C/A | 36 | 46.8 | 31 | 47 | 0.86 | 0.34 | 2.16 | ||
| C/C | 17 | 22.1 | 13 | 19.7 | 0.68 | 0.22 | 2.11 | ||
| A/A | 24 | 31.2 | 22 | 33.3 | 1 | 0.622 | 1 | ||
| C/A-C/C | 53 | 68.8 | 44 | 66.7 | 0.8 | 0.34 | 1.91 | ||
| A/A-C/A | 60 | 77.9 | 53 | 80.3 | 1 | 0.548 | 1 | ||
| C/C | 17 | 22.1 | 13 | 19.7 | 0.74 | 0.28 | 1.98 | ||
| A/A-C/C | 41 | 53.2 | 35 | 53 | 1 | 0.971 | 1 | ||
| C/A | 36 | 46.8 | 31 | 47 | 1.01 | 0.46 | 2.23 | ||
| Additive | 77 | 53.8 | 66 | 46.2 | 0.83 | 0.47 | 1.45 | 0.505 | 1 |
| rs4803222 | |||||||||
| G/G | 33 | 42.9 | 33 | 50.8 | 1 | 0.909 | 1 | ||
| C/G | 39 | 50.6 | 26 | 40 | 0.84 | 0.36 | 1.92 | ||
| C/C | 5 | 6.5 | 6 | 9.2 | 0.84 | 0.2 | 3.56 | ||
| G/G | 33 | 42.9 | 33 | 50.8 | 1 | 0.662 | 1 | ||
| C/G-C/C | 44 | 57.1 | 32 | 49.2 | 0.84 | 0.38 | 1.86 | ||
| G/G-C/G | 72 | 93.5 | 59 | 90.8 | 1 | 0.904 | 1 | ||
| C/C | 5 | 6.5 | 6 | 9.2 | 0.92 | 0.23 | 3.67 | ||
| G/G-C/C | 38 | 49.4 | 39 | 60 | 1 | 0.713 | 1 | ||
| C/G | 39 | 50.6 | 26 | 40 | 0.86 | 0.39 | 1.91 | ||
| Additive | 77 | 54.2 | 65 | 45.8 | 0.88 | 0.48 | 1.64 | 0.694 | 1 |
| rs368234815 | |||||||||
| TT/TT | 34 | 44.2 | 24 | 37.5 | 1 | 0.872 | 1 | ||
| TT/ΔG | 28 | 36.4 | 27 | 42.2 | 1.22 | 0.5 | 2.95 | ||
| ΔG/ΔG | 15 | 19.5 | 13 | 20.3 | 1.27 | 0.43 | 3.72 | ||
| TT/TT | 34 | 44.2 | 24 | 37.5 | 1 | 0.604 | 1 | ||
| TT/ΔG-ΔG/ΔG | 43 | 55.8 | 40 | 62.5 | 1.24 | 0.55 | 2.76 | ||
| TT/TT-TT/ΔG | 62 | 80.5 | 51 | 79.7 | 1 | 0.775 | 1 | ||
| ΔG/ΔG | 15 | 19.5 | 13 | 20.3 | 1.16 | 0.43 | 3.1 | ||
| TT/TT-ΔG/ΔG | 49 | 63.6 | 37 | 57.8 | 1 | 0.771 | 1 | ||
| TT/ΔG | 28 | 36.4 | 27 | 42.2 | 1.13 | 0.5 | 2.54 | ||
| Additive | 77 | 54.6 | 64 | 45.4 | 1.14 | 0.68 | 1.92 | 0.624 | 1 |
DNA samples from 143 newborns with congenital infection by ZIKV, with or without CZS, were genotyped for IFNAR1 SNPs rs2257167, rs2843710, rs2834202, and rs17875834; and IFNL SNPs rs8109886, rs12979860 rs8099917, rs4803222, and rs368234815. Data were adjusted considering the trimester of the first symptoms or asymptomatic ZIKV infections and the percentage of the African and European ancestry of each individual. The total number of genotyped samples for each SNP may vary due to genotype miscalling. Major allele was used as baseline. Odds ratio (OR) with 95% confidence interval (CI) and p-values. We conducted type I error adjustment of multiple comparisons by the False Discovery Rate (FDR) method.