Analysis of transcripts from the ndhA operon in the sot1-3 mutant. (a) Schematic illustration of the Arabidopsis ndhA operon. ndhH, ndhA, ndhI, ndhG, ndhE, psaC, and ndhD are cotranscribed from the ndhA operon. The position of each transcript is diagrammed below the operon model, annotated with its length in kilonucleotides. Spliced transcripts are illustrated with a dashed line in the place of the ndhA intron. Probes a to h are shown below the operon model. (b) RNA gel blot analysis of transcripts from the ndhA operon. Ten micrograms of total leaf RNA isolated from 12-day-old wild-type (WT), sot1-3, and sot1-3com plants was analyzed using an RNA gel blot hybridization. The transcript size is shown in kilonucleotides to the left of each panel. An ethidium bromide staining of the 25S rRNA is shown below each blot as a loading control.