Figure 5.

Post hoc analysis of DEGs of CTNS^−/− ciPTECs. (A) The first step of the pipeline is a schematic representation of the MANTRA output, with the prototype ranked list (PRL) of genes potentially up- or downregulated by each compound; then, lists of genes are organized in order to have at the top those modulated from the highest number of compounds; finally, it was selected upregulated genes, and simultaneously modulated from at least four compounds, which have the downregulated counterpart in the list of DEGs obtained from transcriptome, and vice versa. Identified genes are analyzed with web application Metascape. (B) Network of enriched terms where each node represents an enriched term that is colored by its cluster ID and the thicker of edge link represents their similarity. (C) The bar graph lists the top 11 clusters with their representative enriched terms; color scale is proportional to the statistical significance of the analysis.