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. 2021 Nov 30;22(23):12940. doi: 10.3390/ijms222312940

Figure 2.

Figure 2

Detection of annealing of ATP aptamer with its complementary DNA, and the ATP-triggering dissociation of ATP aptamer from its complementary DNA strand. (A) Quenching-based analysis was used for annealing detection, and ATP triggering dissociates the ATP aptamer from its complementary DNA strand. Error bars indicate SD. (n = 3). (B) Gel electrophoresis assay confirms that ATP aptamer hybridized with its complementary DNA. Lane 1: Unannealed DNA strand contains AS1411 aptamer, lane 2: Unannealed DNA strand contains CRO, lane 3: formed AS1411–ATP aptamer chimera, lane 4: formed CRO–ATP aptamer chimera.