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Effect of different concentrations of Con A on ROS-positive bull spermatozoa (%) incubated with different concentrations of Con A under control conditions (without menadione) and oxidative stress conditions (100 µM menadione). a, b, c: differences within control or oxidative stress between different concentrations of Con A. x, y: differences within a given concentration of Con A between control or oxidative stress (n = 6). Data are expressed as mean ± SEM; significance, p < 0.05.
