Table 1.
Identified proteins showing changes in oxPTMs caused by inhibition of 2-Cys PRDXs with Con A and/or oxidative stress.
| Spot No. | Protein Name | Gene Name | Accession Number |
Calculated MW/pI | Protein Score/Number of Peptides | No. of Cysteines |
|---|---|---|---|---|---|---|
| 101 | Hyaluronidase | SPAM1 | F1MTV1 NP_001008413.3 |
63268/8.79 | 244/2 | 18 |
| 102 | Hyaluronidase | SPAM1 | F1MTV1 NP_001008413.3 |
63268/8.79 | 353/3 | 18 |
| 103 | Pyruvate kinase | PKM | A5D984 | 58482/7.96 | 215/2 | 10 |
| 104 | T-complex protein 1 subunit beta | CCT2 | Q32BHO | 57781/6.18 | 187/2 | 6 |
| 183 | Aconitate hydratase, mitochondrial | ACO2 | A0A3Q1M6K6 XP_005909496.1 |
85481/7.67 | 164/1 | 13 |
| 184 | Aconitate hydratase, mitochondrial | ACO2 | A0A3Q1M6K6 XP_005909496.1 |
85481/7.67 | 164/1 | 13 |
| 185 | Aconitate hydratase, mitochondrial | ACO2 |
P20004 XP_005909496.1 |
86047/7.87 | 244/1 | 13 |
| 286 | T-complex protein 1 subunit gamma | CCT3 |
Q3T0K2 ELR54279.1 |
61118/6.38 | 470/3 | 10 |
| 292 | Succinate dehydrogenase (ubiquinone) flavoprotein subunit, mitochondrial | SDHA | G3MY67 XP_027375926.1 |
74101/7.55 | 906/10 | 19 |
| 304 | Pyruvate kinase | PK | A5D984 | 58482/7.96 | 220/2 | 10 |
| 324 | Calicin | CCIN |
Q28068 XP_005908650.1 |
67873/8.49 | 414/4 | 18 |
| 341 | Carboxypeptidase Q | CPQ | A0A3Q1LSW9 XP_005215732.1 |
52902/5.83 | 356/3 | 1 |
| 344 | 60 kDa chaperonin | HSPD1 | F1MUZ9 XP_015313986.1 |
61110/5.71 | 330/4 | 3 |
| 347 | 60 kDa chaperonin | HSPD1 | F1MUZ9 XP_015313986.1 |
61110/5.71 | 1010/7 | 3 |
| 349 | Carboxypeptidase Q | CPQ | A0A3Q1MFF3 | 49530/5.52 | 188/2 | 5 |
| 394 | ATP synthase subunit alpha | ATP5F1A | F1MLB8 XP_019842301.1 |
59767/9.21 | 399/2 | 2 |
| 397 | ATP synthase subunit alpha | ATP5F1A | F1MLB8 XP_019842301.1 |
59767/9.21 | 496/4 | 2 |
| 422 | Acrosin | ACR | G5E5C6 | 46149/9.64 | 185/2 | 12 |
| 431 | Alpha-enolase | ENO1 |
Q9XSJ4 NP_776474.2 |
47639/6.37 | 291/3 | 6 |
| 444 | Fructose-bisphosphate aldolase | ALDOA | A6QLL8 | 39925/8.45 | 223/2 | 9 |
| 449 | Glyceraldehyde-3-phosphate dehydrogenase, testis-specific | GAPDHS | Q2KJE5 | 43659/8.32 | 152/2 | 5 |
| 454 | Cytochrome b-c1 complex subunit 1, mitochondrial | UQCRC1 | P13272 | 29813/9.04 | 393/3 | 5 |
| 481 | Fructose-bisphosphate aldolase | ALDOA | A6QLL8 XP_006070551.2 |
39925/8.45 | 283/3 | 9 |
| 483 | Citrate synthase, mitochondrial | CS | Q29RK1 | 51968/8.16 | 378/4 | 4 |
| 484 | Phosphoglycerate mutase 2 | PGAM2 |
Q32KV0 NP_001033200.1 |
28838/8.99 | 203/2 | 3 |
| 488 | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADM |
Q3SZB4 NP_001068703.1 |
46943/8.31 | 505/4 | 7 |
| 542 | Zona pellucida binding protein | ZPBP | F1N369 | 40313/9.14 | 312/2 | 15 |
| 559 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | PDHB | P11966 | 39443/6.21 | 271/3 | 6 |
| 612 | F-actin-capping protein subunit beta | CAPZB | A0A3Q1MG13 XP_005203140.1 |
34782/5.59 | 336/2 | 7 |
| 620 | L-lactate dehydrogenase A-like 6B | LDHAL6B | Q3T056 | 42022/8.91 | 379/4 | 8 |
| 627 | L-lactate dehydrogenase A-like 6B | LDHAL6B |
Q3T056 NP_001030352.1 |
42022/8.91 | 379/4 | 8 |
| 725 | Cytochrome b5 heme-binding domain-containing protein | CYB5D1 | A0A3Q1LQK0 | 26704/5.36 | 194/3 | 1 |
| 775 | ATP synthase subunit d, mitochondrial | ATP5PD | P13620 | 18738/5.99 | 436/5 | 1 |
| 801 | Tubulin alpha-3 chain isoform X3 | TUBA3 | XP_024833647.1 | 46605/4.99 | 828/7 | 9 |
| 802 | Tubulin beta-4B chain | TUBB4B | NP_001029835.1 | 50255/4.79 | 498/6 | 8 |