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. 2021 Nov 23;22(23):12659. doi: 10.3390/ijms222312659

Figure 2.

Figure 2

TNF-α, IL-17A, and IFN-γ synergistically induce IL23Ap19 and EBI3 expressions in NHEKs. (A–D) Normal human epidermal keratinocytes (NHEKs) were stimulated with (A,B) TNF-α (50 ng/mL), IL-17A (50 ng/mL), or IFN-γ (50 ng/mL), and (C,D) triple cytokine combinations of TNF-α, IL-17A, IFN-γ, IFN-α, IL-27 and/or IL-17C for 24 h. The relative mRNA expressions of IL23Ap19 and EBI3 to GAPDH were analyzed with quantitative real-time PCR (qPCR). ** p < 0.01, *** p < 0.001, T, TNF-α; 17A, IL-17A; G, IFN-γ.