Skip to main content
. 2021 Nov 23;22(23):12659. doi: 10.3390/ijms222312659

Figure 3.

Figure 3

Topical treatment by dexamethasone, vitamin D3, and acitretin suppress IL23Ap19 and EBI3 expressions in NHEKs. Normal human epidermal keratinocytes (NHEKs) were stimulated with the triple combination of TNF-α (50 ng/mL), IL-17A (50 ng/mL), and IFN-γ (50 ng/mL), and simultaneously treated with (A,B) dexamethasone (10-8,10-7, or 10-6 M), (C,D) 1,25(OH)2 vitamin D3 (10-8,10-7, or 10-6 M), or (E,F) acitretin (10-8,10-7, or 10-6 M) for 24 h. The relative mRNA expressions of IL23Ap19 and EBI3 to GAPDH were analyzed with quantitative real-time PCR (qPCR). ** p < 0.01, *** p < 0.001. T, TNF- α; 17A, IL-17A; G, IFN-γ; Triple, T+17A+G; DEX, dexamethasone; VitD, 1,25(OH)2 vitamin D3; Aci, acitretin.