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. 2021 Nov 23;22(23):12659. doi: 10.3390/ijms222312659

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Proteins detected by immunoprecipitation with tildrakizumab or anti-human EBI3 antibody and LC-Ms/Ms. Culture supernatant of NHEKs stimulated with the triplet mixture of cytokines (50 ng/mL, TNF-α, IL-17A and IFN-γ) for 48 h were immunoprecipitated with (A) humanized anti-human IL-23Ap19 antibody or (B) rabbit anti-human EBI3 antibody and magnetic beads. The detected proteins were analyzed by liquid chromatography–electrospray tandem mass spectrometry (LC-Ms/Ms). The non-specific proteins detected in common with the control were removed.