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. 2021 Dec 1;22(23):13026. doi: 10.3390/ijms222313026

Figure 6.

Figure 6

PCSK9 does not change the expression of chemokine receptors and inflammatory cytokines in human macrophages in vitro. Peripheral blood mononuclear cells (PBMCs) isolated from buffy coats were incubated with monocyte-specific antibodies to isolate human monocytes. These monocytes were then cultured with macrophage colony stimulating factor (M-CSF) for seven days, allowing them to differentiate into macrophages. The expression of chemokine receptors CXCR4, CCR8, CCR5, and CCR2 measured using flow cytometry and represented using geometric mean (Geo.Mean) in human macrophages (n = 3–4 replicates) with and without PCSK9 (A) and when exposed to inflammatory stimulus with LPS (B). Inflammatory cytokines IL6 (C) and TNF-α (D) expression measured in the supernatant of the cell culture with and without PCSK9 in an inflammatory environment (n = 3–4 replicates). Bar graphs are representation of mean ± SEM. 2-way ANOVA was used for (A,B), and Mann–Whitney test was used for (C,D) to calculate the statistical differences.