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The role of the mitochondrial pyruvate kinase in gluconeogenesis in rat kidney. The following scenario is proposed: pyruvate (PYR) derived from L-lactate and amino-acids enters mitochondria via its own carrier; once inside the matrix via the mitochondrial pyruvate kinase (PK) phosphoenolpyruvate (PEP) is synthesized, the reaction being made possible by the removal of the newly synthesized PEP which is exported in exchange with PYR via the putative PYR/PEP antiporter.
