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Phasor-FLIM analysis on human islets. Multiphoton FLIM of NAD(P)H in human islets from representative young (24 years old donor) and aged (58 years old donor) human donors. Phasor histograms show the frequency distribution of NAD(P)H lifetimes in young (black) and aged (red) islets. Scale bar = 5 μm. 2G, 2mM glucose. 17G, 17mM glucose. Adapted from Gregg et al. [25].
