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. 2021 Dec 3;22(23):13094. doi: 10.3390/ijms222313094

Figure 4.

Figure 4

Functional analysis of S-palmitoylated proteins affected by the HPD diet performed with the use of REACTOME. Proteins identified as S-palmitoylated in the membrane-enriched liver fraction of mice fed the RD or HPD diet for 4 or 12 weeks were subjected to a STRING-Reactome/REACTOME functional analysis. The analysis was conducted for proteins whose S-palmitoylation extent was up- or down-regulated (the HPDpalm/RDpalm ratio ≥ 1.5 or ≤ 0.67) after 4 (A,B) or 12 weeks (C,D) of feeding. (A,C) Results of the STRING-Reactome analysis of pathway enrichment in S-palmitoylated proteins are presented as the percentage of total observed proteins. The statistical significance of the enrichment within a specific category is also indicated (FDR). In (B,D) proteins involved in “Metabolism” shown in (A,C), respectively, were subdivided into indicated categories with a direct application of the REACTOME database. The data are presented as the percentage of the input “Metabolism” proteins (95 and 84 at 4 and 12 weeks, respectively). Proteins can overlap between subcategories, categories representing less than 5% of all proteins were combined and are shown as “Others”. Complete lists of proteins from each category are shown in Supplemental Table S3, sheet 1 (4 weeks) and sheet 2 (12 weeks).