Skip to main content
. 2021 Dec 3;22(23):13094. doi: 10.3390/ijms222313094

Figure 5.

Figure 5

Influence of HPD diet on the extent of calfacilitin/TLCD1 S-palmitoylation. Mice were fed RD or HPD diet, sacrificed after 4 (A,D) or 12 (B,E) weeks and their livers isolated. Proteins of the membrane-enriched liver fraction were subjected to ABE procedure encompassing treatment with hydroxylamine (HA+) or not (HA-), biotinylation and capture on streptavidin-agarose beads followed by SDS-PAGE and immunoblotting with anti-calfacilitin/TLCD1 (A–C) or anti-flotillin-1 (D–F) antibodies. Upper panels in (A,B,D,E) show S-palmitoylated protein, lower panels, the respective protein present in the input membrane-enriched liver fraction. Pairs of lanes marked (+ and −) represent individual mice. Positions of molecular weight markers are shown on the right. Extent of S-palmitoylation of calfacilitin/TLCD1 (C) and flotillin-1 (F) normalized against their content in the input fraction. Data are mean ± s.d. from 4 RD and 4 HPD mice at 4 weeks and 5 RD and 6 HPD mice at 12 weeks. *, significantly different at p < 0.05 by and one-way Anova with Scheffe’s post-hoc test.