Figure 4.

(A) Human PBMCs were stimulated with upLPS overnight, followed by treatment with Z-VAD, or 0.1 µM, 1 µM or 10 µM apremilast for one hour before nigericin was added. (B) Cell death was measured using a lactate dehydrogenase (LDH) assay after stimulation of PBMCs with upLPS + Nigericin and apremilast at different doses. (C) Human primary keratinocytes were cultured for 24 h before treatment with upLPS overnight, followed by treatment with Z-VAD, or 0.1 µM, 1 µM or 10 µM apremilast two hrs before nigericin or SDS treatment or UVB-irradiation (0.5 J/cm²). After five hrs incubation at 37 °C, the supernatant was collected and IL-1β ELISA was performed. Mean ± SEM of four different healthy individuals is shown.***: p < 0.001, +: with, -: without.