Table 3.

Primers used in this study.

Primers	Sequence (5′→3′)	Restriction Sites	Function
pKD-pqsA-up	CATCTCGAGCCAGGGTCAGTGTCC	Xho I	Luminous reporter construction
pKD-pqsA-down	TGTGGATCCCAGAACCTCGGTCAG	BamH I	Luminous reporter construction
pqsA-up1	CAGGAAACAGCTATGACCATGATTACGAAGTACCTTGGAAGTCGAGC	-	pqsA knockout
pqsA-down1	CAACATGCCCGTTCCTCGGAACAGAACCTCGGTC	-	pqsA knockout
pqsA-up 2	GACCGAGGTTCTGTTCCGAGGAACGGGCATGTTG	-	pqsA knockout
pqsA-down 2	CGACGGCCAGTGCCAAGCTTCGAACGGATCGAAGCTG	-	pqsA knockout
pAK-pqsA-up	TATGAGCTCCCGTGGTTCTTCTCC	Sac I	pqsA complement
pAK-pqsA-down	CGTTCTAGAGCATCAGCATCTCGTC	Xba I	pqsA complement
pAK-pqsR-up	TATTTAGGTGACACTATAGAATACTCAAGCTTGAATAAGGGATGCCTATTC	Hind III	pqsR complement
pAK-pqsR-down	GAATTGTAATACGACTCACTATAGGGCGAATTCGAGCTCCCACGGCCAGCGTC	EcoR I	pqsR complement