FIG. 5.

PaAOX-dependent respiration is not induced in senescent cultures of the wild-type strain. (A) Oxygen uptake measurements of juvenile (10 days), middle-aged (15 days), and senescent cultures of the wild-type strain. Respiration was measured without addition of respiratory inhibitors, after the addition of 1 mM KCN, or after the addition of 4 mM SHAM. (B) Western blot analysis of PaAOX proteins of juvenile (juv.), middle-aged (m.a.), and senescent (sen.) cultures of the wild-type strain and of mutant grisea. In addition, mitochondrial protein preparations of mutant ex1 were analyzed. PaAOX was detected with AOA monoclonal antibodies directed against the AOX of S. guttatum strain Schott and visualized by enhanced chemiluminescence. An antibody directed against the β subunit of the ATPase complex was used as a loading control. (C) Northern blot analysis of PaAox transcription in juvenile and senescent cultures of the wild-type strain and of mutant grisea. The ethidium bromide-stained RNA is shown as a loading control.