FIG. 8.

PaSod2 is a putative target gene of transcription factor GRISEA. RNA was isolated from wild-type and grisea cultures grown with 100 μM CuSO₄ or without supplements. RNA was treated with DNase I to reduce DNA contamination and subsequently reverse transcribed by using an oligo(dT) primer. Reverse-transcribed RNA samples (cDNA) were used as a template. The PaSod2 transcripts were amplified by using primers MnSOD1 and MnSOD2 (see Materials and Methods). The Gpd gene was amplified in the same reaction and served as an internal control (positive control). DNase I-treated RNA (RNA) was included to exclude amplification of DNA (negative control). Lane m, 100-bp size standard. Numbers on the left indicate fragment sizes in kilobase pairs.