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The activity of PaSOD2 is reduced in senescent cultures of the wild-type strain. Proteins of two independent juvenile (juv.) and two senescent (sen.) cultures were isolated, as well as proteins from a juvenile strain grown for 4 h on 100 μM paraquat (+ Pq) or for 4 days on 100 μM MnCl2 (+ Mn2+). These proteins were subjected to native polyacrylamide gel electrophoresis. SOD activity was determined by staining of the gel.
