REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-BROX	Atlas Antibodies	Cat# HPA031445; RRID: AB_10602697
Mouse monoclonal anti-HSP90α/β	Santa Cruz Biotechnology	Cat# sc-13119; RRID: AB_675659)
Rabbit polyclonal anti-CHMP7	Proteintech	Cat# 16424-1-AP; RRID: AB_2079500
Mouse monoclonal anti-GFP (clones 7.1 and 13.1)	Roche	Cat# 11814460001; RRID: AB_390913
Rabbit polyclonal anti-HA	Antibodies Online	Cat# ABIN100176; RRID: AB_10779560
Rabbit polyclonal anti-UFD1L	Proteintech	Cat# 10615-1-AP; RRID: AB_2213944
Rabbit polyclonal anti-53BP1	Novus Biologicals	Cat# NB100-304; RRID: AB_10003037
Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3)	Cell Signalling	Cat##9718
Rabbit polyclonal anti-Lamin B1	Abcam	Cat# ab16048; RRID: AB_443298
Secondary donkey anti-rabbit Alexa Fluor 555	ThermoFisher Scientific	Cat# A-31572; RRID: AB_162543
Secondary goat anti-mouse (H+L DyLight™ 680 4X PEG Conjugate)	Cell Signaling Technology	Cat# 5470; RRID: AB_10696895
Secondary goat anti-mouse (H+L DyLight™ 800 4X PEG Conjugate)	Cell Signaling Technology	Cat# 5257; RRID: AB_10693543
Secondary goat anti-rabbit (H+L DyLight™ 800 4X PEG Conjugate)	Cell Signaling Technology	Cat# 5151; RRID: AB_10697505
Secondary goat anti-mouse (HRP-linked)	Cell Signaling Technology	Cat# 7076; RRID: AB_330924
Secondary goat anti-rabbit (HRP-linked)	Cell Signaling Technology	Cat# 7074; RRID: AB_2099233
Bacterial and virus strains
BL21	New England Biolabs	C2530H
Chemicals, peptides, and recombinant proteins
Polyethylenimine	Polysciences	Cat# 23966-1
Polybrene	Merck Millipore	Cat# TR-1003-G
Puromycin	Sigma-Aldrich	Cat# P8833
G418	Thermo Fisher Scientific	Cat# 10131035
Lipofectamine 3000	Thermo Fisher Scientific	Cat# L3000008
RNAiMAX	Thermo Fisher Scientific	Cat# 13778150
Dharmafect-1	Horizon Discovery	Cat# T-2001-02
Chlorophenol red-B-D-galactopyranoside (CPRG)	Sigma-Aldrich	Cat# 10884308001
Reversine	Sigma-Aldrich	Cat# R3904
SiR-actin	Spirochrome	Cat# CY-SC001
Hoechst 33258	Sigma-Aldrich	Cat# 861405
Isopropyl β-d-1-thiogalactopyranoside (IPTG)	Generon	Cat# IB0168-25G
Glutathione sepharose 4B beads	GE Healthcare Life Sciences	Cat# 17075601
cOmplete protease inhibitor cocktail	Sigma-Aldrich	Cat# 000000011697498001
GFP-Trap M	ChromoTek	Cat#GTM-20
Eeyarestatin I	Sigma-Aldrich	Cat# E1286
Kifunensine	Sigma-Aldrich	Cat# K1140
Epoxy resin	TAAB	Cat# T028
Uranyless stain	TAAB	Cat# S474
Reynolds lead citrate	TAAB	Cat# L037
Prolong Diamond Antifade Mountant	Thermo Fisher Scientific	Cat# P36965
(S)-3’-amino Blebbistatin	Cambridge Bioscience Ltd	Cat# CAY24170
Y-27632 dihydrochloride	Sigma-Aldrich	Cat# Y0503
Critical commercial assays
ABI High-Capacity cDNA Reverse Transcription Kit	Thermo Fisher Scientific	Cat# 4368814
Experimental models: cell lines
HT1080	ATCC	Cat# CRL-7951; RRID: CVCL_0317
HeLa	ATCC	Cat# CRM-CCL-2; RRID: CVCL_0030
HEK 293T	ATCC	Cat# CRL-3216; RRID: CVCL_0063
HT1080δLaminA/C	De Vos laboratory (Antwerp University)	N/A
Experimental models: organisms/strains
Y190 Yeast Strain	Harper et al., 1993	N/A
Oligonucleotides
siNT: ON-TARGETplus Non-targeting Control siRNA	Horizon Discovery (Dharmacon)	D-001810-01-50
siBROX: Hs_C1orf58_3 FlexiTube siRNA	Qiagen	SI04234300
siCHMP7: 5’-GGGAGAAG AUUGUGAAGUUdTdT-3’	Ventimiglia et al., 2018	Horizon Discovery (Dharmacon) Custom siRNA
siNesprin-2.1: 5’-GAGUGUCG GAGGGAACUAAUU-3’	Jayo et al., 2016	Horizon Discovery (Dharmacon) Custom siRNA
siNesprin-2.2: 5’-GAAGAAAAG GUGCAUGUUAUU-3’	Jayo et al., 2016	Horizon Discovery (Dharmacon) Custom siRNA
siUFD-1: 5’-GAGGCAGA UUCGUCGCUUUdTdT-3’	Olmos et al., 2015	Horizon Discovery (Dharmacon) Custom siRNA
Recombinant DNA
pCMS28 mCherry-NLS	Ventimiglia et al, 2018	N/A
pCMS28 mCherry-BAF	This paper	N/A
pNG72 GFP-L-BROXr	This paper	N/A
pNG72 GFP-L-BROXr C408S	This paper	N/A
pCMS28 mCherry-Emerin	Ventimiglia et al, 2018	N/A
pNG72 CHMP4B-L-GFP	Ventimiglia et al, 2018	N/A
YFP-LAP2β	Carlton et al.,2012	N/A
pNG72 GFP-Emerin	This paper	N/A
pNG72 GFP-NLS	Ventimiglia et al, 2018	N/A
pGEX Empty	Agromayor et al. 2009	N/A
pGEX BROX WT	This paper	N/A
pGEX BROX L350A	This paper	N/A
pGEX BROX H204A	This paper	N/A
pCR3.1 GFP-CHMP4B	Ventimiglia et al, 2018	N/A
pCR3.1 GFP-empty	Ventimiglia et al, 2018	N/A
pCR3.1 hmN2GSR29-33	This paper	N/A
pNG72 GFP-L-BROXr L350A	This paper	N/A
KT7 BROX WT	This paper	N/A
KT7 BROX E137A	This paper	N/A
KT7 BROX H144A	This paper	N/A
KT7 BROX R145A	This paper	N/A
KT7 BROX H204A	This paper	N/A
KT7 BROX L208A	This paper	N/A
KT7 BROX L208/212D	This paper	N/A
KT7 BROX L212D	This paper	N/A
KT7 BROX K322A	This paper	N/A
KT7 BROX Y348A	This paper	N/A
KT7 BROX Y348A	This paper	N/A
KT7 BROX L350A	This paper	N/A
KT7 BROX I354A	This paper	N/A
HB18 CHMP4B	Martin-Serrano et al, 2003	N/A
HB18 CHMP5 C-terminal truncation	This paper	N/A
HB18 Nesprin-2	This paper	N/A
pNG72 GFP-SOUBA	This paper	N/A
HA-Ubiquitin	Agromayor and Martin-Serrano, 2006	N/A
pCR3.1 FLAG-BROX WT	This paper	N/A
pCR3.1 FLAG-BROX H204A	This paper	N/A
pCR3.1 GFP-hmN2G	This paper	N/A
pCR3.1 FLAG-BROX L350A	This paper	N/A
Software and algorithms
FIJI	https://fiji.sc/	N/A
Prism 9	GraphPad	N/A
Imaris 9	Bitplane	N/A
AutoQuant X3	Media Cybernetics	N/A
UCSF Chimera	https://www.cgl.ucsf.edu/chimera/	N/A