Figure 2.

U87-derived neurospheres response to TGF-β can be inhibited by EGCG. (A) U87 glioblastoma neurospheres were cultured as described in the Materials and Methods section, and representative phase contrast pictures were taken. Spheroids were treated or not with 10 nM TGF-β or in combination with 30 μM EGCG for 18 h; total RNA extracted and RT-qPCR analyses were performed to assess (B) cancer stemness and (C) EMT gene expression levels. Probability values of less than 0.05 were considered significant and an asterisk identifies such significance.