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. 2021 Nov 30;22(23):13006. doi: 10.3390/ijms222313006

Figure 3.

Figure 3

SNAIL induction by concanavalin A involves a TGF-β receptor-mediated signaling axis component. (A) Serum starved U87 glioblastoma cells were treated with increasing concentrations of TGF-β in the presence or not of 30 μM EGCG, or 10 μM galunisertib for 24 h. SNAIL, fibronectin and GAPDH protein expression were then assessed by immunoblotting using the respective cell lysates. (B) Serum-starved U87 glioblastoma cells were treated with 30 μg/mL of concanavalin A (ConA) for 24 h in the presence or not of 30 μM EGCG or 10 μM galunisertib (Galu) for 24 h. SNAIL and GAPDH protein expression were then assessed by immunoblotting using the respective cell lysates. (C) Total RNA was extracted from treated cells, and RT-qPCR was performed to assess SNAIL gene expression as described in the Materials and Methods section. Data are means ± SD from three independent experiments. Probability values of less than 0.05 were considered significant and an asterisk identifies such significance.