Figure 4.

MT1-MMP silencing represses TGF-β- and concanavalin A-mediated induction of SNAIL. Transient siRNA-mediated gene silencing was performed in U87 glioblastoma cells transfected with siScrambled, siSNAIL, and siMT1-MMP. Serum-starved cells were next treated with 10 nM TGF-β or 30 μM ConA for 24 h. (A) The conditioned media were harvested and subjected to gelatin zymography to assess proMMP-2 activation into MMP-2, whereas cell lysates were subjected to immunoblotting of SNAIL and MT1-MMP catalytic and hinge forms for protein expression. (B) Total RNA was also extracted from the respective above conditions, and RT-qPCR was performed to monitor MT1-MMP and SNAIL gene expression. Data are means ± SD from three independent experiments. Probability values of less than 0.05 were considered significant and an asterisk identifies such significance.