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. 2021 Nov 30;22(23):13006. doi: 10.3390/ijms222313006

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Concanavalin A and TGF-β share common signaling axis. U87 glioblastoma cells were treated with 10 nM TGF-β or 30 μM ConA for the indicated times, and cell protein lysates were harvested. Long time-course was performed from 0 to 120 min in (A) to monitor Smad2, Smad3, and STAT3 protein status, whereas a short time-course was performed from 0 to 10 min in (B) to monitor Src phosphorylation status. Scanning densitometric analysis was performed of a representative experiment for (C) TGF-β treatment and (D) concanavalin A treatment.