Co-exposure of MDA-MB-231 cells to Dox and MSC CM did not affect cell migration. (A) The effect of CM2D or CM3D in combination with Dox (100 nM) on the migration of MDA-MB-231 cells at the 20 and 30 h time points was evaluated by scratch assay. Cell migration is represented as percentage (mean ± SD, n = 3–6) of wound closure at the defined time points. (B) Representative images of scratch assays of MDA-MB-231 cells incubated with or without MSC CM (control) immediately after the scratches were made (0 h) and after 20 and 30 h. Magnification 4×, scale bar = 100 μm. CM2D, conditioned medium derived from 2D cultures; CM3D, conditioned medium derived from 3D cultures; Dox, doxorubicin.