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Co-exposure of MDA-MB-231 cells to CM3D or CM2D maintained the anti-invasion effect of Dox. The effect of CM2D or CM3D in combination with Dox (100 nM) on the chemoinvasion ability of MDA-MB-231 cells was evaluated by transwell assay. Results are expressed in percentage to control (mean ± SD, n = 4–6). Grid line represents 100% cell viability (control). CM2D, conditioned medium derived from 2D cultures; CM3D, conditioned medium derived from 3D cultures; Dox, doxorubicin.
