Table 1.
Morphological evaluation of zebrafish larvae at 120 hpf in the medium control group, solvent control groups (EMS + 0.5% DMSO and EMS + 1% DMSO) and test groups (CBZ—E-CBZ). Only parameters showing abnormalities are mentioned in the tables. EMS: embryo solution; DMSO: dimethyl sulfoxide; Hpf: hours post fertilization; Exp: experiment. * p < 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.
| Deviation Tail | Oedema | Malformation Head | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 120 hpf Experiment CBZ/E-CBZ |
# | Coagulation | No Hatching | Curve | Tissue Deviation |
Head | Pericardium | Yolk | Deviation Shape Head | Deviation Mouth | Deviation Eye | Deviation Pigmentation |
Swim Bladder Not Inflated |
| EMS | 1 | 2/20 | 2/20 | 2/20 | 1/20 | ||||||||
| 2 | |||||||||||||
| EMS 0.5% DMSO | 1 | 1/20 | 1/20 | 1/20 | |||||||||
| 2 | 1/20 | 1/20 | 2/20 | ||||||||||
| EMS 1% DMSO | 1 | 1/20 | 2/20 | ||||||||||
| 2 | 1/20 | 1/20 | |||||||||||
| CBZ 31.25 µM | 1 | 8/20 ** | |||||||||||
| 2 | 2/20 | ||||||||||||
| CBZ 85 µM | 1 | 1/20 | 7/20 ** | ||||||||||
| 2 | 2/20 | 1/18 | 1/18 | 3/18 | 2/18 | 1/18 | 2/18 | ||||||
| CBZ 250 µM | 1 | 5/20 * | 2/20 | 5/20 * | 3/20 | 4/20 | 5/20 * | 19/20 **** | 16/20 **** | ||||
| 2 | 1/20 | 2/20 | 4/20 | 3/20 | 3/20 | 3/20 | 8/20 ** | 18/20 **** | 11/20 ** | ||||
| E-CBZ 250 µM | 1 | 1/20 | 1/20 | 1/20 | 1/20 | 3/20 | |||||||
| 2 | 1/20 | 1/20 | 1/20 | 6/20 * | |||||||||
Ratios depicted in bold are statistically significant compared to the solvent control group.