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. 2021 Dec 2;22(23):13052. doi: 10.3390/ijms222313052

Figure 4.

Figure 4

Olfml3 deletion attenuated TGFβ-induced microglial immunosuppression. (A) Secretion of colony stimulating factor-1 (CSF-1) was attenuated in Olfml3-/- microglia relative to isogenic control microglia in all conditions; * p < 0.05, ** p < 0.01. (B) While exposure to β1 increased secretion of granulocyte–macrophage colony stimulating factor (GM-CSF) in isogenic control microglia, secretion was reduced in Olfml3-/- microglia; * p < 0.05. (C) The mRNA levels of the pro-inflammatory genes Nos2 and H2ab1 were increased in Olfml3 microglia relative to isogenic control cells following treatment with TGFβ; * p < 0.05, ** p < 0.01. (D) Secretion of CD95, a potent inducer of cytotoxic T cell apoptosis, increased in isogenic control microglia following exposure to β1 and β3, but was undetectable in the media of Olfml3-/- microglia across all conditions; * p < 0.05, ** p < 0.01. Comparisons based on one-way ANOVA with Tukey’s Multiple Comparison Test. Bars represent group mean with standard error of the mean (SEM); data represent one of three independent experiments.