Figure 3.

Application of FAP-tPDT in monolayer culture of primary lesional SSc skin fibroblasts cultured on different substrates. Primary skin fibroblasts were isolated and cultured on either plastic (A) or collagen type 1 (B) and subsequently seeded on plastic or collagen type 1 to determine the dose response of PS-FAP antibody construct in these conditions after exposure to light. For plastic cultured cells the LD₅₀ of the tPDT construct was 0.25 nM and 1 nM for cells seeded on plastic and collagen respectively. For collagen cultured cells, the LD₅₀ of the tPDT construct was 0.5 nM and 1 nM respectively. (n = 4 replicates per donor). * = p < 0.05, *** = p < 0.001.