TABLE 2.
Limit of detection of DNA spiked into water of a nested PCR using Gram-stain-specific bacterial primer pairsa
| Species (n) | Limit of detection with primer pair:
|
|
|---|---|---|
| N6R-NF | P2F-NR | |
| Gram negative | ||
| E. coli (2) | 10 fg | NA |
| K. pneumoniae (3) | 10 fg | NA |
| S. marcescens (3) | 100 fg | NA |
| H. influenzae (2) | 10 fg | NA |
| P. mirabilis (3) | 10 fg | NA |
| P. aeruginosa (3) | 10 fg | NA |
| Gram positive | ||
| S. aureus (3) | NA | 100 fg |
| S. epidermidis (3) | NA | 100 fg |
| S. pyogenes (2) | NA | 100 fg |
| S. faecalis (3) | NA | 100 pg |
| S. viridans (2) | NA | 1 pg |
| S. pneumoniae (3) | NA | 1 pg |
| P. acnes (5) | NA | 10 ng |
| B. cereus (3) | NA | 1 pg |
a
NA, not amplified. Numbers in parentheses indicate the number of strains tested.