Figure 4.

Suppression of Ca²⁺ signals in cortical astrocytes in response to the application of 0.5 µg/mL SeNPs in the presence of a vesicular secretion blocker bafilomycin A1 (A, Baf A1, 10 µM), tetanus toxin (B, TeNT, 100 µg/mL), an inhibitor of Ca²⁺-dependent vesicular fusion, apyrase (C, 30 U/mL), an enzyme that breaks down ATP, P2Y receptor antagonist MRS-2179 (D, 30 µM), combination MRS-2179 with P2X receptor antagonist TN-ATP (E, 10 µM), and connexin blocker carbenoxolone (F, CBX, 100 µM). Here, Ca²⁺ signals of cells in one experiment are presented.